Multifunctional properties of the transmembrane LPxTG-motif protein derived from Limosilactobacillus reuteri SH-23

The LPxTG-motif protein is an important transmembrane with high hydrophilicity and stability, as evidenced by its stress tolerance adhesion ability. In this study, a novel esterase activity (LEP) was expressed, the multifunctional properties such were also investigated. When cocultured Limosilactobacillus reuteri (L. reuteri) SH-23, ability of L. SH-23 to HT-29 cells improved, further found relating potential target Pyruvate kinase M1/2 (PKM) cells. addition, protein, LEP can promote hydrolysis bovine milk lipids activity, enhanced in presence Zn2+ Mn2+ at pH 7. Furthermore, polyunsaturated fatty acids (PUFA) linoleic acid eicosapentaenoic increase during hydrolyzing process. These unique provide comprehensive understanding function PUFAs releasing derived from shed light on beneficial effect Lactobacillus strain colonization gastrointestinal tract.